論工具的重要性——看同行是如何利用HT2800Tfa論文的

更新時間：2022-07-18 點擊次數：1575

Hello小伙伴大家好，今天我們來聊聊工具的重要性。《論語·衛靈公》中有“工欲善其事必先利其器"的說法，我們現在常說的巧婦難為無米之炊引申自陸游《老學庵筆記》卷三：“僧曰：巧婦安能作無面湯餅乎？"。可見工具的重要性，儀器設備對于科研及質檢人員來說是尤為重要的，有好的儀器設備不一定能發高分論文，但沒有儀器設備沒有實驗數據支撐，一定發不了高分論文，做一個“裝備控"是非常有必要的。

HT2800T是意大利HTA公司研發生產的一款多功能氣相色譜自動進樣器，可以與眾多進口品牌的GC或GCMS聯用，實現操作的自動化升級，完成液體進樣、頂空進樣及SPME固相微萃取進樣，滿足您的實驗不同應用需求。

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簡潔的操作，清晰的觸摸屏，安裝方便不同應用模式切換僅需5min，不同儀器之間拆裝也僅需5min。

用事實說話，看“歪果仁"是如何利用HT2800T搞科研fa論文的。

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應用一：《用金屬氧化物氣體傳感器S3裝置鑒別特級初榨橄欖油的質量和地理來源》

特級初榨橄欖油(EVOO)因其價格昂貴、營養價值高，是最常見的摻假食品之一，歐盟也出臺了一項立法，以維護一些與生產區域有關的EVOOs商標，因此，監測和確保EVOO的質量和地理來源很重要。利用HT2800T頂空模式與氣相色譜儀聯用，結合其他金屬傳感器系統完成初榨橄欖油的色譜分析及在線檢測，根據所得結果判斷EVOO的質量及地理來源。

涉及進樣及色譜方法原文如下：

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The complex set of data acquired from the S3 sensors response to the volatile organic compounds originating from the EVOO headspace were analyzed through a m*riate analysis PCA and ANN. The measurements were performed in an autosampler HT2800T (HTA srl, Brescia, Italy) . For this, 5mL of olive oil samples were enclosed in 20mL vials and placed randomly in the autosampler. The sample headspace was drawn out from the vial and injected at 1mL/min into a chromatographic air flow (50 sccm). The injection time was 2min and the recovery time was 5min.

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應用二：《蘋果酒的品質取決于必需無機鹽》

在發酵前向酵母中添加適量的無機鹽有助于促進酵母的生長改進整個工藝流程，使得產品質量更高，為了評估蘋果酒必須補充無機鹽(NH₄)₂SO₄, MgSO₄，(NH₄)₃PO₄對釀酒參數、抗氧化活性、總多酚含量的影響用各種酵母菌株發酵的揮發性蘋果酒化合物。文中檢測揮發性有機物使用了PE的氣相色譜與HTA的HT2800T，SPME進樣模式。

涉及進樣及色譜方法原文如下：

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Analysis of Volatile Compounds

A sample of 2mL was placed into a 15-mL headspace vial, and 50µL of internal standard solution (5mg/L of ethyl nonanoate) was added. Then, the SPME (solid-phase microextraction) fiber (85µm Carboxen Polydime thylsiloxane, Supelco, St. Louis, MO, USA) was placed in the headspace above the sample, and the vial was incubated for 30min at 40℃. The fiber was subjected to thermal desorption in a gas chromatograph injector at 250℃.

The chromatographic separation was carried out on a Clarus 580 apparatus (PerkinElmer, Waltham,MA, USA) and a Crossbond dimethyl polysiloxane 60 m, 0.25mm, 1.4µm film thickness column. The carrier gas flow (He) was 2mL/min, with the following

temperature program: 35℃, 6min;8℃/min up to 180℃; 12℃/min up to220℃; 25min. The detector and dispenser temperature was 250℃. An HT2800T autosampler (HTA Brescia, Italy) was used,and PerkinElmer Total Chrom 6.3.2 software (PerkinElmer, Waltham, MA, USA) was used to integrate the results.

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應用三：《在蘋果酒生產的不同階段必需氧對釀酒參數、抗氧化活性和揮發性化合物的影響》

微氧作用可能會影響成品酒jing飲料的質量，不同發酵階段的微氧作用對不同酵母菌株發酵的揮發性蘋果酒的釀造參數、抗氧化活性、總多酚含量影響不同。經微氧處理的蘋果酒提取物濃度較高，酸度較高，效力較低。必須氧化在發酵過程中降低了揮發性成分的濃度。發酵過程中必須菌的氧化作用對蘋果酒中萜類化合物的濃度有不同程度的影響。文中檢測揮發性物質同樣也是利用了HT2800T的SPME進樣模式。

涉及進樣及色譜方法原文如下：

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Analysis of Volatile Compounds

The volatile compounds were determined using a modified Grützman et al. method. A sample of 2mL was placed into a 15mL headspace vial, and 50µL of an internal standard solution (5mg/L of ethyl nonanoate) was added. Then a solid-phase microextraction (SPME) fiber was placed in the headspace above the sample, and the vial was incubated for 30 minutes at 40℃. The fiber was subjected to thermal desorption in a gas chromatographic injector at 250℃.

The chromatographic separation was carried out on a Clarus 580 apparatus equipped with a flame ionization detector (FID) and a Crossbond dimethylpolysiloxane column (60m in length, 0.25 mm of inner diameter, 1.4µm of film thickness). The carrier gas flow (He) was 2mL/min, and the temperature program was: 35℃, 6 min;8℃/min up to 180℃; 12℃/min up to 220℃; 25 min. The inlet and FID temperature was 250℃.

An HT2800T autosampler (HTA Brescia, Italy) was used, and PerkinElmer Total Chrom 6.3.2 software(PerkinElmer, Walthman, MA, USA) was used to integrate the results. Identification was performed based on the retention times compared with those of the standards for the volatile compounds, and quantification was carried out by internal standardization, using 4-methyl-2-pentanol (alcohols) and ethyl nonanoate (esters) as the internal standards. The standard solutions were prepared in synthetic wine (20g/L sucrose, 5g/L tartaric acid, 10% ethanol). All experiments were performed in triplicate.

HT2800T

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好的儀器不僅可以幫我們完成科研或質檢工作；提高工作效率；提供準確可信的實驗數據；fa論文都離不開她。你想做“裝備控"嗎？

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論工具的重要性——看同行是如何利用HT2800Tfa論文的

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